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dc.contributor.authorJahis, Besek Mariam
dc.contributor.authorIlham, Zul
dc.contributor.authorSupramani, Sugenendran
dc.contributor.authorSohedein, Mohamad Nor Azzimi
dc.contributor.authorIbrahim, Mohamad Faizal
dc.contributor.authorAbd-Aziz, Suraini
dc.contributor.authorRowan, Neil
dc.contributor.authorWan-Mohtar, Wan Abd Al Qadr Imad
dc.identifier.citationMohamad Jahis, B.M.; Ilham, Z.; Supramani, S.; Sohedein, M.N.A.; Ibrahim, M.F.; Abd-Aziz, S.; Rowan, N.; Wan-Mohtar, W.A.A.Q.I. (2022) Ganodiesel: A New Biodiesel Feedstock from Biomass of the Mushroom Ganoderma lucidum. Sustainability. 14, 10764.
dc.description.abstractThere is a pressing demand for new sustainable eco-friendly approaches to producing green energy worldwide. This study represents the novel production of biodiesel feedstock from the medicinal mushroom Ganoderma lucidum QRS 5120 using state-of-the-art biotechnology tools. Response surface methodology (RSM) was used to enhance G. lucidum production in a repeated-batch fermentation strategy. By referring to the broth replacement ratio (BRR) and broth replacement time point (BRTP), RSM that was formulated using a central composite design (CCD) resulted in a signifi cant model for all tested variables, which are exopolysaccharide (EPS), endopolysaccharide (ENS) and biomass, with BRR (%) of 60, 75 and 90, and BRTP (days) of 11, 13 and 15. The model was validated using the optimised conditions, and the results showed 4.21 g/L of EPS (BRR 77.46% and BRTP 12 days), 2.44 g/L of ENS (BRR 60% and BRTP 12.85 days), and 34.32 g/L of biomass (BRR 89.52% and BRTP 10.96 days) were produced. Biomass produced from the G. lucidum was subsequently used as feedstock for biodiesel production. Approximately 20.36% of lipid was successfully extracted from the dried G. lucidum biomass via a solvent extraction and subsequently converted to Ganodiesel through a transesterification process. The Ganodiesel produced fulfilled most of the international standards, i.e., US (ASTM D6751-08) and EU (EN 14214). Overall, this study demonstrates the optimised G. lucidum production and its lipid production as a new biodiesel feedstock.en_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.subjectBioreactor for biomassen_US
dc.subjectGanoderma lucidumen_US
dc.subjectMushroom cultivationen_US
dc.subjectRepeated-batch fermentationen_US
dc.titleGanodiesel: a new biodiesel feedstock from biomass of the mushroom Ganoderma lucidumen_US
dc.contributor.affiliationTechnological University of the Shannon Midlands Midwesten_US
dc.contributor.sponsorThis research was funded by Universiti Malaya International Collaboration Grant ST002- 2022, ST007-2021, European Atlantic Area Neptunus (Project EAPA_576/2018) and Regional Univer sity Network European University (RUN-EU Project)en_US
dc.subject.departmentBioscience Research Institute TUS:MMen_US

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Attribution-NonCommercial-NoDerivatives 4.0 International
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