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dc.contributor.authorBarron, Valerie
dc.contributor.authorNeary, Martin
dc.contributor.authorMohamed, Khalid Merghai Salid
dc.contributor.authorAnsboro, Sharon
dc.contributor.authorShaw, Georgina
dc.contributor.authorO'Malley, Grace
dc.contributor.authorRooney, Niall
dc.contributor.authorBarry, Frank
dc.contributor.authorMurphy, Mary
dc.identifier.citationBarron, V., Neary, M., Mohamed, K.M.S. et al. (2016) Evaluation of the early in vivo response of a functionally graded macroporous scaffold in an osteochondral defect in a rabbit model. Annals of Biomedical Engineering. 44, 1832–1844.
dc.identifier.otherArticles - Materials Research Institute AITen_US
dc.description.abstractCartilage tissue engineering is a multifactorial problem requiring a wide range of material property requirements from provision of biological cues to facilitation of mechanical support in load-bearing diarthrodial joints. The study aim was to design, fabricate and characterize a template to promote endogenous cell recruitment for enhanced cartilage repair. A polylactic acid poly-ε-caprolactone (PLCL) support structure was fabricated using laser micromachining technology and thermal crimping to create a functionally-graded open pore network scaffold with a compressive modulus of 9.98 ± 1.41 MPa and a compressive stress at 50% strain of 8.59 ± 1.35 MPa. In parallel, rabbit mesenchymal stem cells were isolated and their growth characteristics, morphology and multipotency confirmed. Sterilization had no effect on construct chemical structure and cellular compatibility was confirmed. After four weeks implantation in an osteochondral defect in a rabbit model to assess biocompatibility, there was no evidence of inflammation or giant cells. Moreover, acellular constructs performed better than cell-seeded constructs with endogenous progenitor cells homing through microtunnels, differentiating to form neo-cartilage and strengthening integration with native tissue. These results suggest, albeit at an early stage of repair, that by modulating the architecture of a macroporous scaffold, pre-seeding with MSCs is not necessary for hyaline cartilage repair.en_US
dc.relation.ispartofAnnals of Biomedical Engineeringen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland*
dc.subjectFunctionally gradeden_US
dc.subjectPolylactic acid-ε-polycaprolactoneen_US
dc.subjectCartilage repairen_US
dc.subjectNeotissue formationen_US
dc.titleEvaluation of the early in vivo response of a functionally graded macroporous scaffold in an osteochondral defect in a rabbit model.en_US
dc.contributor.sponsorEuropean Union’s 7th Framework Programme under grant agreement no. HEALTH- 2007-B-223298 (PurStem), Science Foundation Ireland (grant number 09/SRC/B1794), Wellcome Trust Biomedical Vacation Scholarships grant number WTD004448 and the Irish Government’s Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007-2013.en_US
dc.rights.accessOpen Accessen_US
dc.subject.departmentMaterials Research Instituteen_US

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