Show simple item record

dc.contributor.authorRowan, Neil J.
dc.contributor.authorCaldow, George
dc.contributor.authorGemmell, Curtis G.
dc.contributor.authorHunter, Iain S.
dc.identifier.citationRowan, J.J., Caldow, G., Gemmell, C.G., Hunter, I.S. (2003).Production of diarrheal enterotoxins and other potential virulence factors by veterinary isolates of Bacillus species associated with nongastrointestinal infections. Applied and Environmental Microbiology. v.69 (4), pp.2372-2376. doi: 10.1128/aem.69.4.2372-2376.2003en_US
dc.identifier.otherFaculty of Science & Health -Nursing and Healthcare-Articlesen_US
dc.description.abstractWith the exceptions of Bacillus cereus and Bacillus anthracis, Bacillus species are generally perceived to be inconsequential. However, the relevance of other Bacillus species as food poisoning organisms and etiological agents in nongastrointestinal infections is being increasingly recognized. Eleven Bacillus species isolated from veterinary samples associated with severe nongastrointestinal infections were assessed for the presence and expression of diarrheagenic enterotoxins and other potential virulence factors. PCR studies revealed the presence of DNA sequences encoding hemolysin BL (HBL) enterotoxin complex and B. cereus enterotoxin T (BceT) in five B. cereus strains and in Bacillus coagulans NB11. Enterotoxin HBL was also harbored by Bacillus polymyxa NB6. After 18 h of growth in brain heart infusion broth, all seven Bacillus isolates carrying genes encoding enterotoxin HBL produced this toxin. Cell-free supernatant fluids from all 11 Bacillus isolates demonstrated cytotoxicity toward human HEp-2 cells; only one Bacillus licheniformis strain adhered to this test cell line, and none of the Bacillus isolates were invasive. This study constitutes the first demonstration that Bacillus spp. associated with serious nongastrointestinal infections in animals may harbor and express diarrheagenic traditionally linked to toxigenic B. cereusen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofApplied and Environmental Microbiologyen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland*
dc.subjectFood poisoningen_US
dc.subjectFood contaminationen_US
dc.titleProduction of diarrheal enterotoxins and other potential virulence factors by veterinary isolates of Bacillus species associated with nongastrointestinal infections.en_US
dc.identifier.doidoi: 10.1128/aem.69.4.2372-2376.2003
dc.rights.accessOpen Accessen_US
dc.subject.departmentFaculty of Science and Healthen_US

Files in this item


This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland