Show simple item record

dc.contributor.authorSun, Yi-Cheng
dc.contributor.authorChen, Yan-Cheng
dc.contributor.authorTian, Zhe-Xian
dc.contributor.authorLi, Feng-Mei
dc.contributor.authorWang, Xin-Yue
dc.contributor.authorZhang, Jing
dc.contributor.authorXiao, Zhen-Long
dc.contributor.authorLin, Min
dc.contributor.authorGilmartin, Niamh
dc.contributor.authorDowling, David N.
dc.contributor.authorWang, Yi-Ping
dc.identifier.citationSun, Y. C., Chen, Y. C., Tian, Z. X., Li, F. M., Wang, X. Y., Zhang, J., . . . Wang, Y. P. (2005). Novel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in China. Applied and Environmental Microbiology, 71(8), 4771-4776. doi:10.1128/aem.71.8.4771-4776.2005en_US
dc.description.abstractGlyphosate has been used globally as a safe herbicide for weed control. It inhibits 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (AroA), which is a key enzyme in the aromatic amino acid biosynthetic pathway in microorganisms and plants. A Pseudomonas putida strain, 4G-1, was isolated from a soil heavily contaminated by glyphosate in China. Its AroA-encoding gene (aroA) has been cloned, sequenced, and expressed in Escherichia coli. Phylogenetic analysis revealed that this AroA belongs neither to class I nor to class II AroA enzymes. When compared with E. coli AroA, 4G-1 AroA shows similar values for Km[PEP], Km[S3P], and specific enzyme activity. Moreover, 4G-1 AroA exhibits high tolerance to glyphosate, which indicates a protein with a high potential for structural and functional studies of AroA in general and its potential usage for the generation of transgenic crops resistant to the herbicide.en_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofAmerican Society for Microbiologyen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland*
dc.titleNovel AroA with high tolerance to glyphosate, encoded by a gene of Pseudomonas putida 4G-1 isolated from an extremely polluted environment in Chinaen_US
dc.contributor.sponsorThis research was supported by grants from the Ministry of Science and Technology of China (The National Transgenic Research and Industrialization Special Foundation of China, grants J00-A-010 and JY03-A-05), the National Natural Science Foundation of China (grant 39925017), the Higher Education Authority of Ireland PRTLI programme, and EU contracts QLK3-CT2000-00164 and QLK3-CT-2001-00101.en_US
dc.subject.departmentDepartment of Science & Health - IT Carlowen_US

Files in this item


This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland