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dc.contributor.authorVillacieros, Marta
dc.contributor.authorWhelan, Clare
dc.contributor.authorMackova, Martina
dc.contributor.authorMolgaard, Jesper
dc.contributor.authorSanchez-Contreras, Maria
dc.contributor.authorLloret, Javier
dc.contributor.authorAguirre de Carcer, Daniel
dc.contributor.authorOruezabal, Roke I.
dc.contributor.authorBolanos, Luis
dc.contributor.authorMacek, Thomas
dc.contributor.authorKarlson, Ulrich
dc.contributor.authorDowling, David N.
dc.contributor.authorMartın, Marta
dc.contributor.authorRivilla, Rafael
dc.date.accessioned2018-10-24T20:27:44Z
dc.date.available2018-10-24T20:27:44Z
dc.date.copyright2004-12-01
dc.date.issued2004
dc.identifier.citationVillacieros, M., Whelan, C., Mackova, M., Molgaard, J., Sanchez-Contreras, M., Lloret, J., . . . Rivilla, R. (2005). Polychlorinated biphenyl rhizoremediation by Pseudomonas fluorescens F113 derivatives, using a Sinorhizobium meliloti nod system to drive bph gene expression. Applied and Environmental Microbiology, 71(5), 2687-2694. doi:10.1128/aem.71.5.2687-2694.2005en_US
dc.identifier.issn1098-5336
dc.identifier.urihttps://research.thea.ie/handle/20.500.12065/2368
dc.description.abstractRhizoremediation of organic chemicals requires high-level expression of biodegradation genes in bacterial strains that are excellent rhizosphere colonizers. Pseudomonas fluorescens F113 is a biocontrol strain that was shown to be an excellent colonizer of numerous plant rhizospheres, including alfalfa. Although a derivative of F113 expressing polychlorinated biphenyl (PCB) biodegradation genes (F113pcb) has been reported previously, this strain shows a low level of bph gene expression, limiting its rhizoremediation potential. Here, a high-level expression system was designed from rhizobial nod gene regulatory relays. Nod promoters were tested in strain F113 by using β-galactosidase transcriptional fusions. This analysis showed that nodbox 4 from Sinorhizobium meliloti has a high level of expression in F113 that is dependent on an intact nodD1 gene. A transcriptional fusion of a nodbox cassette containing the nodD1 gene and nodbox 4 fused to a gfp gene was expressed in the alfalfa rhizosphere. The bph operon from Burkholderia sp. strain LB400 was cloned under the control of the nodbox cassette and was inserted as a single copy into the genome of F113, generating strain F113L::1180. This new genetically modified strain has a high level of BphC activity and grows on biphenyl as a sole carbon and energy source at a growth rate that is more than three times higher than that of F113pcb. Degradation of PCBs 3, 4, 5, 17, and 25 was also much faster in F113L::1180 than in F113pcb. Finally, the modified strain cometabolized PCB congeners present in Delor103 better than strain LB400, the donor of the bph genes used.en_US
dc.formatPDFen_US
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofApplied and Environmental Microbiologyen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/ie/*
dc.titlePolychlorinated biphenyl rhizoremediation by Pseudomonas fluorescens F113 derivatives, using a Sinorhizobium meliloti nod system to drive bph gene expression.en_US
dc.typeArticleen_US
dc.contributor.grantnoBIO4-CT97-2227en_US
dc.contributor.grantnoQLK3-CT-2001-00101en_US
dc.contributor.grantnoBIO2003-03412en_US
dc.contributor.sponsorThis work was funded by European Commission grants BIO4-CT97-2227 and QLK3-CT-2001-00101. D.N.D. was also funded in part by the HEA PRTLI TSR Strand 3 and SFI programs, Ireland. M. Martín was also funded by the Spanish Ministry for Science and Technology (grant BIO2003-03412) and is the recipient of a Ramón y Cajal contract from MCYT. R.I.O. is the recipient of a predoctoral fellowship from the Basque Country Government.en_US
dc.description.peerreviewyesen_US
dc.identifier.urlhttps://doi.org/10.1128/AEM.71.5.2687-2694.2005en_US
dc.rights.accessinfo:eu-repo/semantics/openAccessen_US
dc.subject.departmentDepartment of Science & Health - IT Carlowen_US


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