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dc.contributor.authorMoreira, António S
dc.contributor.authorHorgan, Finbarr G.
dc.contributor.authorMurray, Tomás E.
dc.contributor.authorKakouli-Duarte, Thomais
dc.date.accessioned2018-08-17T14:01:21Z
dc.date.available2018-08-17T14:01:21Z
dc.date.copyright2013-07-11
dc.date.issued2013
dc.identifier.citationMoreira, A. S., Horgan, F. G., Murray, T. E., & Kakouli-Duarte, T. (2013). Bumblebee (Hymenoptera: Apidae) sample storage for a posteriori molecular studies: Interactions between sample storage and DNA-extraction techniques. European Journal of Entomology, 110(3), 419-425. doi:10.14411/eje.2013.056en_US
dc.identifier.issn1210-5759
dc.identifier.urihttps://research.thea.ie/handle/20.500.12065/2332
dc.description.abstractA global decline in pollinator abundance and diversity has demanded increased research attention to the ecology and genetics of bumblebees. However, as progressively more restrictions are placed on sampling for insects, researchers are increasingly obliged to use archival specimens collected for purposes other than genetic analyses. In this study we assessed the suitability, for population genetic studies, of popular, low-cost methods for preservation and storage of bumblebee specimens. Specimens of Bombus terrestris L. were held under six storage regimes for up to two years. DNA was extracted from the samples using three extraction protocols and the quality of the DNA was examined using PCR amplification of a mitochondrial and a nuclear gene. All extraction and storage methods provided sufficient DNA for successful PCR amplification. However, samples preserved in acetone or at freezing temperatures yielded the highest DNA concentrations. DNA yields from pinned specimens at room temperature declined over time, particularly when using standard extraction techniques. DNA concentrations were significantly lower from specimens preserved in 70% ethanol compared to all other extraction techniques and declined linearly over the two years of storage. These results indicate that two of the most popular insect storage methods (pinning and storage in ethanol) should be avoided for the long-term preservation of genetic material for future studies. We suggest that optimal insect preservation methods should be incorporated into research protocols in order to best capitalise on limited collection opportunities.en_US
dc.formatPDFen_US
dc.language.isoenen_US
dc.publisherInstitute of Entomologyen_US
dc.relation.ispartofEuropean Journal of Entomologyen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/ie/*
dc.subjectBombus terrestrisen_US
dc.subjectacetoneen_US
dc.subjectcommercial kiten_US
dc.subjectDNA qualityen_US
dc.subjectentomological pinsen_US
dc.subjectfreezingen_US
dc.subjectphenol-extractionen_US
dc.subjectHymenopteraen_US
dc.subjectApidaeen_US
dc.titleBumblebee (Hymenoptera: Apidae) sample storage for a posteriori molecular studies: Interactions between sample storage and DNA-extraction techniques.en_US
dc.typeArticleen_US
dc.contributor.grantnoRSF 06 348en_US
dc.contributor.sponsorFunding for this research was provided by the Irish Department of Agriculture, Food and the Marine as part of a research stimulus grant (RSF 06 348).en_US
dc.description.peerreviewyesen_US
dc.identifier.urlhttp://dx.doi.org/10.14411/eje.2013.056en_US
dc.rights.accessinfo:eu-repo/semantics/openAccessen_US
dc.subject.departmentenviroCORE - IT Carlowen_US


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Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland