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Characterization of norepinephrine-evoked inward currents in interstitial cells isolated from the rabbit urethra.
Freshly dispersed interstitial cells from the rabbit urethra were studied by using the perforated-patch technique. When cells were voltage clamped at -60 mV and exposed to 10 microM norepinephrine (NE) at 80-s intervals, ...
Mediation by nitric oxide of neurogenic relaxation of the urinary bladder neck muscle in sheep.
Mechanical recordings were made in vitro from circularly oriented strips of the bladder neck muscle of sheep. In the absence of drugs, electrical field stimulation at frequencies of 0.2-1 Hz evoked clear-cut relaxations ...
Characterization of T-type calcium current and its contribution to electrical activity in rabbit urethra.
Rabbit urethral smooth muscle cells were studied at 37 degrees C by using the amphotericin B perforated-patch configuration of the patch-clamp technique, using Cs(+)-rich pipette solutions. Two components of current, with ...
Pacemaker activity in urethral interstitial cells is not dependent on capacitative calcium entry.
The aim of the present study was to investigate the properties and role of capacitative Ca(2+) entry (CCE) in interstitial cells (IC) isolated from the rabbit urethra. Ca(2+) entry in IC was larger in cells with depleted ...
Role of IP(3) in modulation of spontaneous activity in pacemaker cells of rabbit urethra.
Isolated interstitial ("pacemaker") cells from rabbit urethra were examined using the perforated-patch technique. Under voltage clamp at -60 mV, these cells fired large spontaneous transient inward currents (STICs), averaging ...
Hyperpolarisation-activated inward current in isolated sheep mesenteric lymphatic smooth muscle.
Freshly isolated sheep lymphatic smooth muscle cells were studied using the perforated patch-clamp technique. Hyperpolarisation with constant-current pulses caused a time-dependent rectification evident as a depolarising ...
Ca2+ current and Ca(2+)-activated chloride current in isolated smooth muscle cells of the sheep urethra.
solated sheep urethral cells were studied using the perforated patch clamp technique (T = 37 degrees C). Depolarizing steps ranging from -40 to -10 mV evoked an inward current that peaked within 10 ms and a slower inward ...
Tetrodotoxin-sensitive sodium current in sheep lymphatic smooth muscle.
Fast inward currents were elicited in freshly isolated sheep lymphatic smooth muscle cells by depolarization from a holding potential of -80 mV using the whole-cell patch-clamp technique. The currents activated at voltages ...
Mediation of excitatory neurotransmission by the release of ATP and noradrenaline in sheep mesenteric lymphatic vessels.
Spontaneous isometric contractions were measured in rings of sheep mesenteric lymphatics. Field stimulation at short pulse widths increased the frequency of spontaneous contractions and this response was blocked by 3 x ...
Outward currents in smooth muscle cells isolated from sheep mesenteric lymphatics
The patch-clamp technique was used to measure membrane currents in isolated smooth muscle cells dispersed from sheep mesenteric lymphatics. Depolarizing steps positive to -30 mV evoked rapid inward currents followed by ...